UNIX, ssh, and scp

The first challenge is going to be to log in to a remote computer and transfer some files around. This is one of those things that most people simply don’t do when using Mac OS X or Windows, so it might be a bit strange. Follow the cookbook examples and we’ll get you through it, though!

Some background

Most of the computation for this course is going to be done on someone else’s computer system, not the laptop in front of you.

This is for a couple of reasons: first and foremost, many laptops are not that powerful or that fast, compared to server-style computers. (That’s because laptops are usually optimized for battery life and size rather than for speed or computation.)

Second, most research tools are built for UNIX operating systems and do not have a graphical interface, which means they neither run well on Windows nor can be used easily by someone that doesn’t know UNIX.

Third, UNIX systems are much easier to control from other computers; for example, I do almost all my work on a computer server iny my lab at Michigan State, but I spend some of my time working on a server at Caltech and I also do some work on a cluster of computers across campus at MSU. Apart from network speed issues, there’s no difference in working on a UNIX computer at Caltech or on one at Michigan State. This is an advantage when you need to use multiple computers to get a job done!

And fourth, it’s easy to rent UNIX computer systems these days through ‘cloud computing’, which we’ll show you in the next tutorial.

So, what this tutorial is going to teach you is how to interact with remote computers by first logging into them, and second, by transferring files to them.

ssh is a mechanism for logging into other computers over the network.

scp is a file copy program built on top of ssh.

Getting started

There are different instructions for Mac users and Windows users, unfortunately.

For Windows, see: Using SSH/SCP on Windows.

For Mac OS X, see: Using SSH/SCP on Mac OS X in the Terminal app.

Digression: Some shell commands that will come in handy

The UNIX shell is as complete a way to interact with computers and files on the computer as the windowing interface (or graphical interface) that you’re used to on Windows and Macs. You just have to type a lot more!

(Remember, you can copy and paste from this document into the shell window!)

Here are a few useful commands for you to try out.

To get a list of files, do:

%% ls

To make a directory:

%% mkdir $dirname

where you replace ‘$dirname’ with the name of the directory.

To see your current directory:

%% pwd

To change directories:

%% cd $dirname

There are lots and lots of UNIX tutorials available. Here is a UNIX tutorial for beginners that might come in handy...

Transferring 3rd-party files to your remote system

One thing we’re going to be doing a lot of is transferring files around. You can do this in a large variety of ways – you’ve already seen ‘scp’. The problem is that a lot of times we’re going to be working on a remote machine and we will want to copy a file from, say, NCBI to that machine, but we won’t want that file to be downloaded to our own computer first, because we don’t need it there. (Think about the situation where you’re at home, and you need to download the human genome. Much better to directly transfer it to the big computer at work then the laptop in front of you!)

Using curl to retrieve files from Web and FTP sites

The simplest way to do this for files from NCBI is to use a program called ‘curl’, which grabs files from the Web and saves them to wherever curl is running. For example, to download the mouse protein RefSeq set from ftp://ftp.ncbi.nih.gov/refseq/M_musculus/mRNA_Prot/, you would type:

%% curl -O ftp://ftp.ncbi.nih.gov/refseq/M_musculus/mRNA_Prot/mouse.protein.faa.gz

Try it while logged into an EC2 machine and changed into your directory – this will download a 10 mb file directly to it, without going through your laptop!

%% cd $dirname
%% curl -O ftp://ftp.ncbi.nih.gov/refseq/M_musculus/mRNA_Prot/mouse.protein.faa.gz

the ‘-O’ option tells curl to guess the filename from the last component of the URL – in this case, ‘mouse.protein.faa’. It will save whatever it downloads to that filename.

If you forget to use the ‘-O’, then you’ll get a lot of gibberish on your screen – that’s actually the contents of the file, dumped to your screen. Hit CTRL-C and start over!

Retrieving scripts and source code for the NGS class

We’ve also made a bunch of files available for the NGS course, through bitbucket. We’ll tell you more about this next week, but for now try running this command to retrieve them all. On your EC2 machine, type

%% hg clone http://bitbucket.org/ctb/ngs-course

This will now create a directory ‘ngs-course’ with a bunch of files underneath it. You can see what files there are by doing:

%% ls -R ngs-course

As we continue to update the scripts and files for this course, you may need to update your copy of ngs-course. To do that, type

%% cd ngs-course

to go into the ngs-course/ directory and then type

%% hg pull -u

– this will “pull” the latest changes we’ve made to the ngs-course from our repository into your local copy.

We’ll talk more about hg later; it is part of a package called Mercurial – hg, mercury, get it? hah hah – for managing and collaborating on changes to scripts and files.

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